For the first time, we report the click here characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae.LUCO was converted to fatty acid methyl esters (FAMEs).We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs.
Our investigations for the analysis of LUCO samples represent noble features of glycerides.A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols.The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID).
The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.
91%) and stearic acid (6.01%).A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).
The structural elucidation and thermal click here stability of LUCO were studied by FT-IR, 1H NMR, 13C NMR spectroscopic techniques and TGA-DSC, respectively.We also measured the cytotoxicity of LUCO.